Toby Flood has been released to play for Leicester this weekend - but Lee Dickson is still part of England's plans for the clash with Italy.
Flood made his comeback from knee ligament damage for the Tigers last Saturday and he spent the last two days training with the national team.
But interim coach England coach Stuart Lancaster has decided fly-half Flood needs another game under his belt before making his Test return and released him to play for Leicester against Exeter.
Charlie Hodgson was limited to non-contact work in training as he recovers from a blow to the shoulder but he is set to retain his place at fly-half for the game at the Stadio Olimpico.
Dickson was initially expected to be sidelined for four weeks after fracturing a bone in his hand during his Test debut off the bench in England's 13-6 win against Scotland.
But the Northampton scrum-half, described as "Lazarus" by coach Andy Farrell, trained with the squad on Tuesday and is one of three scrum-halves retained in the 24-man squad that will fly to Rome.
Joe Simpson has joined Ben Youngs and Dickson in the trimmed-down squad, replacing Gloucester wing Charlie Sharples as the only change to the squad that travelled to Edinburgh last weekend.
Lancaster will finalise his 22-man match-day squad to face Italy on Thursday morning, before the squad travel to Rome.
Sharples, who missed out on the final 22 last week, joins Flood as one of eight players released for Aviva Premiership action this weekend, along with Dave Attwood, Matt Banahan, Calum Clark, Karl Dickson, Lee Mears and Thomas Waldrom.
The New Zealand All Blacks are wearing this new rugby jersey during their training sessions. Get in the Gear.
Price: $74.99 Buy at World Rugby ShopHeineken Cup holders Leinster will defend their title against Ulster on May 19. Get in the Gear!
Price: $89.99 Buy at World Rugby ShopUlster will look to win their first Heineken Cup title since 1998/99 on May 19 against Leinster.
Price: $19.99 Buy at World Rugby ShopA World Rugby Shop exclusive in the U.S., Stade Francais rugby gear. Make a statement.
Price: $74.99 Buy at World Rugby ShopmiCoach is being introduced in the game of Rugby. The latest addition to the soft ground collection is the adizero RS7 Pro II Rugby Boot!
Price: $199.99 Buy at World Rugby Shop
Rugby Match Ticker
Follow LIVE rugby action from around the globe with Rugby Rugby!
The World Rugby Shop has the best selection of official and team rugby gear from around the globe. Shop for your rugby gear today!