The Azzurri begin the campaign in Paris on February 4 against Brunel's home nation France before taking on England a week later at Rome's Stadio Olimpico.
Brunel, who replaced Nick Mallett at the helm in November, has included a total of 22 players that competed in last year's World Cup.
However, experienced quartet Salvatore Perugini, Carlos Del Fava, Mirco Bergamasco and Gonzalo Garcia were surprisingly left out of the squad, with Brunel opting to include uncapped youngsters Alberto De Marchi, Luca Morisi, Angelo Esposito and Giovanbattista Venditti instead.
Aironi flanker Mauro Bergamasco returns to the squad having missed last year's entire tournament with a shoulder injury.
Italy begin training in Rome on Sunday.
Italy squad: Martin Castrogiovanni, Lorenzo Cittadini, Alberto De Marchi, Andrea Lo Cicero, Leonardo Ghiraldini, Tommaso D'Apice, Marco Bortolami, Quintin Geldenhuys, Antonio Pavanello, Cornelius Van Zyl, Robert Barbieri, Mauro Bergamasco, Paul Derbyshire, Simone Favaro, Sergio Parisse, Alessandro Zanni, Edoardo Gori, Fabio Semenzato, Kristopher Burton, Luciano Orquera, Tommaso Benvenuti, Gonzalo Canale, Luca Morisi, Alberto Sgarbi, Angelo Esposito, Roberto Quartaroli, Giulio Toniolatti, Giovanbattista Venditti, Andrea Masi, Luke McLean.
Gilbert has released a new line of rugby cleats. The Gilbert Virtuo 8S is part of the exciting new product. Check it out.
The entire All Blacks apparel line has been updated for 2013/14. Check out the New Zealand All Blacks polo.
The Nike Tiempo is a solid rugby cleat and one of few styles still made from full-grain natural leather.
The Gilbert Blitz 8S rugby cleat is a great cleat at a great price of $69.99. Get a new pair of cleats today.
A cool looking all black rugby cleat with the high performance adidas is known for. Get in the Gear!
Wear the crest of the British and Irish Lions on your t-shirt. A great look for the summer.
The All Blacks Performance t-shirt is black with hints of blue from the training jersey. Very Cool.
The New Zealand All Blacks training jersey for 2013/14. Get in the Gear!
The USA Rugby Pro Alternate rugby jersey is perfect for any fan of the Eagles. Get yours to wear during the summer Test matches.
The NEW All Blacks 2013/14 jersey has arrived at World Rugby Shop. Dare to wear the colors of the All Blacks.