The Sharks ace comes into the starting XV after Zane Kirchener suffered a knee injury in the first Test victory.
Bjorn Basson will take Lambie's spot on the bench, with Werner Kruger also named among the replacements after Coenie Oosthuizen was ruled out for three months.
"We decided against no unnecessary changes as we aim to build some continuity during this season," said coach Heyneke Meyer.
"We still have not had a lot of preparation time, but I'm pleased to have seen how the players have been getting stuck in - on the training field and during our off-field sessions.
"This weekend will be a big challenge yet again against a good England side, but our focus is still on ourselves. We need to show further improvement from last weekend and have to make a step up this weekend.
"We have confidence in all the players in our squad and that is why Pat, Werner and Bjorn have slotted in easily. Everyone knows what is needed this weekend and they have been very sharp at training. We now need to translate that to the match on Saturday."
South Africa: 15 P Lambie; 14 JP Pietersen, 13 J de Villiers (capt), 12 F Steyn, 11 B Habana; 10 M Steyn, 9 F Hougaard; 1 T Mtawarira, 2 B du Plessis, 3 J du Plessis; 4 E Etzebeth, 5 J Kruger, 6 M Coetzee, 7 W Alberts, 8 P Spies.
Replacements: 16 A Strauss, 17 W Kruger, 18 F van der Merwe, 19 K Daniel, 20 R Pienaar, 21 W Olivier, 22 B Basson.
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