The number eight, who was due to captain Wales for a record-equalling 28th time, has had a calf problem and will not be risked ahead of the World Cup next month.
Jones is replaced by Sale Sharks forward Andy Powell, with Jonathan Thomas moving on to the bench.
Flanker Martyn Williams, who wins his 99th cap this weekend, will skipper the side.
Wales coach Warren Gatland names his 30-man World Cup squad on Monday, when Jones is expected to be part of a group that faces pool games against South Africa, Samoa, Namibia and Fiji.
With Matthew Rees out of the tournament through injury and current skipper Sam Warburton rested this weekend, Jones was set to take the reins for a 28th time, equalling Ieuan Evans' Wales captaincy record.
Powell, meanwhile, now has a golden opportunity to press his World Cup claims ahead of Monday's announcement.
He has been out of favour for much of this year, but is still a destructive player when he produces top form.
He moved to Sale from Wasps earlier this summer.
Gilbert has released a new line of rugby cleats. The Gilbert Virtuo 8S is part of the exciting new product. Check it out.
The entire All Blacks apparel line has been updated for 2013/14. Check out the New Zealand All Blacks polo.
The Nike Tiempo is a solid rugby cleat and one of few styles still made from full-grain natural leather.
The Gilbert Blitz 8S rugby cleat is a great cleat at a great price of $69.99. Get a new pair of cleats today.
A cool looking all black rugby cleat with the high performance adidas is known for. Get in the Gear!
Wear the crest of the British and Irish Lions on your t-shirt. A great look for the summer.
$29.99
The All Blacks Performance t-shirt is black with hints of blue from the training jersey. Very Cool.
The New Zealand All Blacks training jersey for 2013/14. Get in the Gear!
The USA Rugby Pro Alternate rugby jersey is perfect for any fan of the Eagles. Get yours to wear during the summer Test matches.
The NEW All Blacks 2013/14 jersey has arrived at World Rugby Shop. Dare to wear the colors of the All Blacks.
