The Newport Gwent Dragons number eight will fly home to have his injury further assessed, with Wales caretaker coach Rob Howley calling up Melbourne Rebels' Gareth Delve from outside his original 34-man squad.
Former Gloucester captain Delve won the last of his 11 caps in 2010, and he was at yesterday's game in a radio commentary capacity.
Wing George North, meanwhile, will undergo intensive treatment on a haematoma to his thigh in a bid to get him fit for the second Test in Melbourne next Saturday.
North was forced off during the first half of Saturday's encounter, and he is crucial to Wales' hopes of squaring the series.
Howley has named his team for Tuesday's appointment with the Brumbies in Canberra, with lock Luke Charteris the only survivor from Brisbane.
Delve will be on the replacements' bench, where his colleagues include Ospreys lock Ian Evans, who arrived in Australia last night following his wedding in Greece.
Evans' Ospreys second-row partner Alun-Wyn Jones will captain the team, taking over from a rested Sam Warburton, while there is another opportunity for 19-year-old Cardiff Blues wing Harry Robinson.
Robinson lit up Wales' 30-21 victory over the Barbarians eight days ago by scoring a dazzling solo try, and he will look to impress on Tuesday, especially with North battling injury.
Howley said: "We are a game down, but we still have all to play for in this Test series and Tuesday night is the perfect opportunity for this squad of players to move on.
"We have one or two bumps and bruises, and we are expecting players to back-up after playing a tough Test match and go out there 72 hours later and play for their country.
"But that is exactly what touring is all about. It will be a test of character for the likes of Luke (Charteris), James (Hook) and Alun-Wyn (Jones), who all made significant contributions on Saturday."
Wales team v Brumbies:
Liam Williams (Scarlets); H Robinson (Cardiff Blues), A Bishop (Ospreys), A Beck (Ospreys), A Brew (Newport Gwent Dragons); J Hook (Perpignan), R Webb (Ospreys); P James (Ospreys), R Hibbard (Ospreys), R Jones (Scarlets), A-W Jones (Ospreys, capt), L Charteris (Newport Gwent Dragons), J Turnbull (Scarlets), J Tipuric (Ospreys), A Shingler (Scarlets).
Replacements: K Owens (Scarlets), R Gill (Saracens), I Evans (Ospreys), G Delve (Melbourne Rebels), Lloyd Williams (Cardiff Blues), R Priestland (Scarlets), A Cuthbert (Cardiff Blues).
Gilbert has released a new line of rugby cleats. The Gilbert Virtuo 8S is part of the exciting new product. Check it out.
The entire All Blacks apparel line has been updated for 2013/14. Check out the New Zealand All Blacks polo.
The Nike Tiempo is a solid rugby cleat and one of few styles still made from full-grain natural leather.
The Gilbert Blitz 8S rugby cleat is a great cleat at a great price of $69.99. Get a new pair of cleats today.
A cool looking all black rugby cleat with the high performance adidas is known for. Get in the Gear!
Wear the crest of the British and Irish Lions on your t-shirt. A great look for the summer.
$29.99
The All Blacks Performance t-shirt is black with hints of blue from the training jersey. Very Cool.
The New Zealand All Blacks training jersey for 2013/14. Get in the Gear!
The USA Rugby Pro Alternate rugby jersey is perfect for any fan of the Eagles. Get yours to wear during the summer Test matches.
The NEW All Blacks 2013/14 jersey has arrived at World Rugby Shop. Dare to wear the colors of the All Blacks.
