Januarie, 29, was initially named in the 49-man party but the 47-cap half-back decided to end his international career and focus on his impending move to French Top 14 newcomers Lyon.
That has seen 27-year-old McLeod called up with the squad set to begin preparations for the upcoming international campaign at an assessment camp in Cape Town from July 5-8.
A South African Rugby Union statement read: "Charl McLeod has been called in to the training squad in place of Ricky Januarie, who has retired from international rugby."
The Stormers players in the party will not attend if their side reach the Super Rugby final.
Coach Peter de Villiers will name his squad for the first game of the Tri Nations on July 9.
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