The 78-cap Wasps back-row forward, who made his Test debut in the 1999 World Cup and was part of the victorious squad in 2003, is one of five players to have been released by manager Martin Johnson.
The Leicester pair of hooker George Chuter and number eight Thomas Waldrom have also been released along with Saracens wing David Strettle and the versatile Gloucester back James Simpson-Daniel.
Johnson has retained a 40-man squad to begin preparations for England's forthcoming back-to-back internationals against Wales.
The decision to cut Worsley and Waldrom, who has been struggling with a calf injury, means the Harlequins captain Chris Robshaw is still pushing for World Cup selection.
Chuter's departure leaves England with three hookers in Dylan Hartley, Steve Thompson and Lee Mears - all of whom are now likely to travel to New Zealand.
Strettle and Simpson-Daniel have been overtaken in the pecking order by Gloucester bolter Charlie Sharples and Harlequins wing Ugo Monye.
Johnson is yet to cut any of his seven props or his five second row players.
Gilbert has released a new line of rugby cleats. The Gilbert Virtuo 8S is part of the exciting new product. Check it out.
The Barbarians are one of the top invitational rugby sides with a long history and classic rugby jersey.
The Nike Tiempo is a solid rugby cleat and one of few styles still made from full-grain natural leather.
The Lions get ready for their matches with this green training jersey. It's what the players wear. Get in the Gear!
A cool looking all black rugby cleat with the high performance adidas is known for. Get in the Gear!
Wear the crest of the British and Irish Lions on your t-shirt. A great look for the summer.
$29.99
The Lions are ready to get their Australia tour underway. They arrive in Perth on Monday.
The New Zealand All Blacks training jersey for 2013/14. Get in the Gear!
The USA Rugby Pro Alternate rugby jersey is perfect for any fan of the Eagles. Get yours to wear during the summer Test matches.
The NEW All Blacks 2013/14 jersey has arrived at World Rugby Shop. Dare to wear the colors of the All Blacks.
