Lock Hendre Fourie is also set to miss out when the squad is named at 7.45pm BST.
Various outlets in the English press have reported that Johnson has decided on a 17-13 split between forwards and backs, with New Zealand-born Wasps centre Flutey the biggest casualty.
Scrum-half Danny Care is ruled out through injury.
Sale's Fourie, who started against Wales in Cardiff, is also set to miss out, along with Harlequins skipper Chris Robshaw.
England play their final warm-up game against Ireland in Dublin on Saturday before opening their World Cup campaign against Argentina.
Gilbert has released a new line of rugby cleats. The Gilbert Virtuo 8S is part of the exciting new product. Check it out.
The entire All Blacks apparel line has been updated for 2013/14. Check out the New Zealand All Blacks polo.
The Nike Tiempo is a solid rugby cleat and one of few styles still made from full-grain natural leather.
The Gilbert Blitz 8S rugby cleat is a great cleat at a great price of $69.99. Get a new pair of cleats today.
A cool looking all black rugby cleat with the high performance adidas is known for. Get in the Gear!
Wear the crest of the British and Irish Lions on your t-shirt. A great look for the summer.
$29.99
The All Blacks Performance t-shirt is black with hints of blue from the training jersey. Very Cool.
The New Zealand All Blacks training jersey for 2013/14. Get in the Gear!
The USA Rugby Pro Alternate rugby jersey is perfect for any fan of the Eagles. Get yours to wear during the summer Test matches.
The NEW All Blacks 2013/14 jersey has arrived at World Rugby Shop. Dare to wear the colors of the All Blacks.
