Matfield and De Villiers will each be out for around 10 days after coming off with a hamstring and rib injury, respectively, during Sunday's 17-16 opening defeat of Wales in Wellington.
The news comes as a further blow to the Springboks, who could yet be forced to replace lock Bakkies Botha due to his struggles with an Achilles problem.
South Africa team doctor Craig Roberts said: "Bakkies hasn't responded as well as we anticipated, but we are still working on it and we haven't made a decision as to how we're going to deal him at this stage."
Asked when he will have to decide on whether to replace Botha, South Africa coach Peter de Villiers said: "Sooner rather than later."
Gilbert has released a new line of rugby cleats. The Gilbert Virtuo 8S is part of the exciting new product. Check it out.
The entire All Blacks apparel line has been updated for 2013/14. Check out the New Zealand All Blacks polo.
The Nike Tiempo is a solid rugby cleat and one of few styles still made from full-grain natural leather.
The Gilbert Blitz 8S rugby cleat is a great cleat at a great price of $69.99. Get a new pair of cleats today.
A cool looking all black rugby cleat with the high performance adidas is known for. Get in the Gear!
Wear the crest of the British and Irish Lions on your t-shirt. A great look for the summer.
$29.99
The All Blacks Performance t-shirt is black with hints of blue from the training jersey. Very Cool.
The New Zealand All Blacks training jersey for 2013/14. Get in the Gear!
The USA Rugby Pro Alternate rugby jersey is perfect for any fan of the Eagles. Get yours to wear during the summer Test matches.
The NEW All Blacks 2013/14 jersey has arrived at World Rugby Shop. Dare to wear the colors of the All Blacks.
